Plasmid

Part:BBa_K4357001:Design

Designed by: Di Yu   Group: iGEM22_UM_Macau   (2022-10-11)


Pasr-XeR-mCherry-pSB1C3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 3879
    Illegal suffix found in sequence at 1831
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3879
    Illegal SpeI site found at 1832
    Illegal PstI site found at 1846
    Illegal NotI site found at 1650
    Illegal NotI site found at 1839
    Illegal NotI site found at 3885
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3879
    Illegal BamHI site found at 899
    Illegal XhoI site found at 854
    Illegal XhoI site found at 1659
    Illegal XhoI site found at 2863
    Illegal XhoI site found at 3755
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 3879
    Illegal suffix found in sequence at 1832
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 3879
    Illegal XbaI site found at 3894
    Illegal SpeI site found at 1832
    Illegal PstI site found at 1846
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Pasr-promoter is act as the core regulator of XeR protein, which is the inward acid proton pump. Also, his-tag and mCherry fluorescent protein is used to monitor the protein expression level in the target organism.


Source

1.https://parts.igem.org/Part:BBa_K1231000 2.https://parts.igem.org/Part:BBa_K4357000 3.https://parts.igem.org/Part:BBa_B0015 4.https://parts.igem.org/Part:BBa_B0034 5.https://parts.igem.org/Part:pSB1C3

References